Application
Western blot analysis of Dynamin expression in mouse brain tissue extract.Application
Western blot analysis of Dynamin I expression in non-transfected 293T: (A), human Dynamin I transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Dynamin expression in EOC 20 (A), NIH/3T3 (B), Sol8 (C) and 3T3-L1 (D) whole cell lysates.Application
Western blot analysis of Dynamin expression in rat brain (A) and mouse brain (B) extracts.| Product Name | Dynamin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 567-866 mapping at the C-terminus of Dynamin II of human origin |
| Modification Notes | Members of the dynamin family, including Dynamin I and Dynamin II, are GTPase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis (1-3). Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression found in testis (1,2). Both dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the dynamins and effectors of their GTPase activity (4,5). The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis (6). Dynamin I appears to be recruited to clathrin coated pits by SH3 domain interaction with amphiphysin, a protein highly expressed in brain (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dynamin I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 567-866 mapping at the C-terminus of Dynamin II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 567-866 mapping at the C-terminus of Dynamin II of human origin |
| Entrez Gene | 1759 |
|---|
Application
Western blot analysis of Dynamin expression in mouse brain tissue extract.
Application
Western blot analysis of Dynamin I expression in non-transfected 293T: (A), human Dynamin I transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Dynamin expression in EOC 20 (A), NIH/3T3 (B), Sol8 (C) and 3T3-L1 (D) whole cell lysates.
Application
Western blot analysis of Dynamin expression in rat brain (A) and mouse brain (B) extracts.| Application Notes | recommended for detection of Dynamin I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
