Application
Western blot analysis of Podocin expression in non-transfected 293T: (A), mouse Podocin transfected 293T: (B) and TE671 (C) whole cell lysates.Application
Western blot analysis of Podocin expression in TE671 (A) and Caki-1 (B) whole cell lysates and rat cerebellum (C), mouse cerebellum (D) and mouse kidney (E) tissue extracts.Application
Western blot analysis of Podocin expression in Caki-1 whole cell lysate (A) and mouse kidney tissue extract (B).| Product Name | Podocin Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 (deletion 30-61) of Podocin of human origin |
| Modification Notes | The onset of autosomal recessive steroid-resistant nephrotic syndrome (SRN1) in humans occurs by early childhood. Characteristics of SRN1 include proteinuria, rapid progression to end-stage renal disease, and focal segmental glomerulo-sclerosis. The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Podocin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 (deletion 30-61) of Podocin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 (deletion 30-61) of Podocin of human origin |
| Entrez Gene | 7827 |
|---|
Application
Western blot analysis of Podocin expression in non-transfected 293T: (A), mouse Podocin transfected 293T: (B) and TE671 (C) whole cell lysates.
Application
Western blot analysis of Podocin expression in TE671 (A) and Caki-1 (B) whole cell lysates and rat cerebellum (C), mouse cerebellum (D) and mouse kidney (E) tissue extracts.
Application
Western blot analysis of Podocin expression in Caki-1 whole cell lysate (A) and mouse kidney tissue extract (B).| Application Notes | recommended for detection of Podocin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
