Application
Western blot analysis of Fyn expression in non-transfected: (A) and human Fyn transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Fyn expression in non-transfected 293: (A), human Fyn transfected 293: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of Fyn expression in non-transfected: (A) and human Fyn transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fyn expression in non-transfected 293T: (A), human Fyn transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized (A) H4 cells and (B) human peripheral blood lymphocytes, showing specificity for the brain isoform of Fyn. Black line histograms represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with Fyn showing membrane staining.Application
Antibodies tested include Fyn (FYN3): (A) and Fyn (15): (B).Application
Western blot analysis of NIH/3T3 cells (A,C,E) and NIH/3T3 cells transfected with a Fyn expression vector (B,D,F) using Fyn (FYN3): (A,B), Fyn (FYN3)-G: (C,D) and Fyn (15): (E,F).| Product Name | Fyn Antibody (FYN3) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Fyn of human origin |
| Modification Notes | Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking. Fyn and Lck kinases play a key role in T-cell antigen receptor (TCR) signaling. The human Fyn gene maps to chromosome 6q21 and encodes a 537 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Fyn p59 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Fyn of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Fyn of human origin |
| Entrez Gene | 2534 |
|---|
Application
Western blot analysis of Fyn expression in non-transfected: (A) and human Fyn transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Fyn expression in non-transfected 293: (A), human Fyn transfected 293: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of Fyn expression in non-transfected: (A) and human Fyn transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fyn expression in non-transfected 293T: (A), human Fyn transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized (A) H4 cells and (B) human peripheral blood lymphocytes, showing specificity for the brain isoform of Fyn. Black line histograms represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with Fyn showing membrane staining.
Application
Antibodies tested include Fyn (FYN3): (A) and Fyn (15): (B).
Application
Western blot analysis of NIH/3T3 cells (A,C,E) and NIH/3T3 cells transfected with a Fyn expression vector (B,D,F) using Fyn (FYN3): (A,B), Fyn (FYN3)-G: (C,D) and Fyn (15): (E,F).| Application Notes | recommended for detection of Fyn p59 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
