Application
Western blot analysis of p38α expression in non-transfected 293T: (A), mouse p38α transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of p38α expression in non-transfected 293T: (A), human p38α transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Antibodies tested include p-p38 (D-8): (A,B) and p38α (C-20): (C,D).Application
Antibodies tested include p38α (N-20): (A,B) and p38α (C-20): (C-F).Application
Western blot analysis of p38α expression in Jurkat (A), A-431 (B) and MCF7 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK rat cells (A) and control DAPI nuclear staining (B).| Product Name | p38α Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of p38α of mouse origin |
| Modification Notes | MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38å, p38∫ and p38©, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38å, p38∫ and p38© are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p38α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of p38&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of p38α of mouse origin |
| Entrez Gene | 1432 |
|---|
Application
Western blot analysis of p38α expression in non-transfected 293T: (A), mouse p38α transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of p38α expression in non-transfected 293T: (A), human p38α transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Antibodies tested include p-p38 (D-8): (A,B) and p38α (C-20): (C,D).
Application
Antibodies tested include p38α (N-20): (A,B) and p38α (C-20): (C-F).
Application
Western blot analysis of p38α expression in Jurkat (A), A-431 (B) and MCF7 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK rat cells (A) and control DAPI nuclear staining (B).| Application Notes | recommended for detection of p38α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
