Application
Western blot analysis of PME-1 expression in non-transfected: (A) and human PME-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), mouse PME-1 transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), mouse PME-1 transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), human PME-1 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of PME-1 expression in A549 (A) and HeLa (B) whole cell lysates.| Product Name | PME-1 Antibody (H-226) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-386 of PME-1 of human origin |
| Modification Notes | Protein phosphatase methylesterase-1 (PME-1) catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. Carboxymethylation and demethylation is a covalent modifcation that regulates the catalytic activity of certain proteins in eukaryotes. Electrostatic interactions that occur at residues or metals in or near the active site can influence the specificity of carboxymethylation and demethylation. PME-1 can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. PME-1 is conserved from yeast to human and contains a motif found in lipases having a catalytic triad-activated serine as their active site nucleophile. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PME-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-386 of PME-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-386 of PME-1 of human origin |
| Entrez Gene | 51400 |
|---|
Application
Western blot analysis of PME-1 expression in non-transfected: (A) and human PME-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), mouse PME-1 transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), mouse PME-1 transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of PME-1 expression in non-transfected 293T: (A), human PME-1 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of PME-1 expression in A549 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of PME-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
