Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IFN-γ expression in Jurkat (A), CCRF-CEM (B), AML-193 (C) and Daudi (D) whole cell lysates.| Product Name | IFN-γ Antibody (H-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 22-166 representing mature interferon-γ (IFN-γ) of human origin |
| Modification Notes | Interferon (IFN)-© is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-© production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-© is inducible by IL-2 , FGF and EGF. The active form of IFN-© is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-© is stabilized by non-covalent interactions through the interface of the helices. IFN-© translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 22-166 representing mature interferon-&gamma (IFN-&gamma ) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 22-166 representing mature interferon-γ (IFN-γ) of human origin |
| Entrez Gene | 3458 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IFN-γ expression in Jurkat (A), CCRF-CEM (B), AML-193 (C) and Daudi (D) whole cell lysates.| Application Notes | recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
