Application
Western blot analysis of IRS-1 expression in non-transfected 293T: (A), human IRS-1 transfected 293T: (B) and A549 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic and nuclear staining of neuronal cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.Application
Western blot analysis of IRS-1 expression in Ramos whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic localization| Product Name | IRS-1 Antibody (H-165) FITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 905-1070 of IRS-1 of human origin |
| Modification Notes | The insulin receptor substrate-1 (IRS-1), a protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 905-1070 of IRS-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 905-1070 of IRS-1 of human origin |
| Entrez Gene | 3667 |
|---|
Application
Western blot analysis of IRS-1 expression in non-transfected 293T: (A), human IRS-1 transfected 293T: (B) and A549 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic and nuclear staining of neuronal cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.
Application
Western blot analysis of IRS-1 expression in Ramos whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic localization| Application Notes | recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
