Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tissue showing cytoplasmic staining of glandular cells.Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of IRS-1 expression in A549 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization using indirect FITC (A) staining and direct Alexa Fluor 488 (B) staining.Application
Western blot analysis of IRS-1 expression in non-transfected control (A) and IRS-1 siRNA transfected (B) HeLa cells. Blot probed with IRS-1 (C-20): . α-actinin (H-2): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.Application
Antibodies tested include IRS-1 (C-20): (A,B) and IRS-1 (C-20)-G: (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue. Note cytoplasmic staining of mucosal epithelia.| Product Name | IRS-1 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IRS-1 of human origin |
| Modification Notes | The insulin receptor substrate-1 (IRS-1), a protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of IRS-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IRS-1 of human origin |
| Entrez Gene | 3667 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tissue showing cytoplasmic staining of glandular cells.
Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of IRS-1 expression in A549 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization using indirect FITC (A) staining and direct Alexa Fluor 488 (B) staining.
Application
Western blot analysis of IRS-1 expression in non-transfected control (A) and IRS-1 siRNA transfected (B) HeLa cells. Blot probed with IRS-1 (C-20): . α-actinin (H-2): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.
Application
Antibodies tested include IRS-1 (C-20): (A,B) and IRS-1 (C-20)-G: (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue. Note cytoplasmic staining of mucosal epithelia.| Application Notes | recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
