Application
Antibodies tested is p-IRS-1 (Tyr 465)-R: (A,B,C) and IRS-1 (A-19): (D,E,F).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.Application
Antibodies tested include p-IRS-1 (Ser 636): (A,B,C) and IRS-1 (A-19): (D,E,F).Application
Antibodies tested include p-IRS-1 (Ser 636): (A,B,C) and IRS-1 (A-19): (D,E,F).Application
Antibodies tested include p-IRS-1 (Ser 374)-R: (A,B,C) and IRS-1 (A-19): (D,E,F).Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.Application
Western blot analysis of IRS-1 expression in A549 (A), Ramos (B) and BJAB (C) whole cell lysates.| Product Name | IRS-1 Antibody (A-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of IRS-1 of human origin |
| Modification Notes | The insulin receptor substrate-1 (IRS-1), a protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of IRS-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of IRS-1 of human origin |
| Entrez Gene | 3667 |
|---|
Application
Antibodies tested is p-IRS-1 (Tyr 465)-R: (A,B,C) and IRS-1 (A-19): (D,E,F).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.
Application
Antibodies tested include p-IRS-1 (Ser 636): (A,B,C) and IRS-1 (A-19): (D,E,F).
Application
Antibodies tested include p-IRS-1 (Ser 636): (A,B,C) and IRS-1 (A-19): (D,E,F).
Application
Antibodies tested include p-IRS-1 (Ser 374)-R: (A,B,C) and IRS-1 (A-19): (D,E,F).
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic localization.
Application
Western blot analysis of IRS-1 expression in A549 (A), Ramos (B) and BJAB (C) whole cell lysates.| Application Notes | recommended for detection of IRS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
