Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Cdc2 p34 expression in IB4 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear localization.| Product Name | Cdc2 p34 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdc2 p34 of human origin |
| Modification Notes | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdc2 p34 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdc2 p34 of human origin |
| Entrez Gene | 983 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Cdc2 p34 expression in IB4 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear localization.| Application Notes | recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
