Application
Western blot analysis of TC-PTP expression in PC-3 whole cell lysate.| Product Name | TC-PTP Antibody (M-115) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 292-406 mapping at the C-terminus of TC-PTP of mouse origin |
| Modification Notes | T-cell protein tyrosine phosphatase (TC-PTP) is a non-transmembrane enzyme. The noncatalytic domain of TC-PTP is alternatively spliced to generate p45TC, which localizes to the nucleus, and p48TC, which contains a hydrophobic C-terminal tail and localizes to the ER. The C-terminal segment of p45TC regulates the activity of the catalytic domain through an intramolecular interaction. The p45TC variant of TC-PTP exits the nucleus upon EGF receptor activation and recognizes the EGF receptor and p52Shc cellular substrates. The p45TC activity almost completely inhibits the EGF-dependent activation of PI 3-kinase and PKB/Akt. In glioblastoma cells, the p45TC variant inhibits the DEGFR-mediated activation of ERK2 and suppresses tumorigenicity in vivo. TC-PTP may play a role in lymphoctye signaling and hematopoietic homeostasis. TC-PTP negatively regulates JAK1 and JAK3 and TC-PTP-deficient mice display gross defects in the hematopoietic system. The gene encoding human TC-PTP maps to chromosome 18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TC-PTP of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 292-406 mapping at the C-terminus of TC-PTP of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 292-406 mapping at the C-terminus of TC-PTP of mouse origin |
| Entrez Gene | 19255 |
|---|
Application
Western blot analysis of TC-PTP expression in PC-3 whole cell lysate.| Application Notes | recommended for detection of TC-PTP of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
