Application
Western blot analysis of THEM2 expression in Hep G2 (A) and HeLa (B) whole cell lysates and mouse kidney (C) and human liver (D) tissue extracts.Application
Western blot analysis of THEM2 expression in Ramos whole cell lysate (A) and mouse liver tissue extract (B).| Product Name | THEM2 Antibody (H-88) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 53-140 mapping at the C-terminus of THEM2 of human origin |
| Modification Notes | Esterases comprise a superfamily of hydrolase enzymes that use water to catalyze the dissociation of an ester into an acid and an alcohol. The thioesterases, a subfamily of esterase proteins, exhibit thiol-specific esterase activity. THEM2 (thioesterase superfamily member 2), also known as HT012 or PNAS-27, is a 140 amino acid proteins that belongs to the thioesterase subfamily of esterase enzymes. Highly expressed in kidney with moderate expression in brain, liver and intestines, THEM2 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. The gene encoding THEM2 maps to chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of THEM2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 53-140 mapping at the C-terminus of THEM2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 53-140 mapping at the C-terminus of THEM2 of human origin |
| Entrez Gene | 55856 |
|---|
Application
Western blot analysis of THEM2 expression in Hep G2 (A) and HeLa (B) whole cell lysates and mouse kidney (C) and human liver (D) tissue extracts.
Application
Western blot analysis of THEM2 expression in Ramos whole cell lysate (A) and mouse liver tissue extract (B).| Application Notes | recommended for detection of THEM2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
