Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferus ducts.Application
Western blot analysis of RARβ expression in U-87 MG whole cell lysate.| Product Name | RARβ Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of RARβ of human origin |
| Modification Notes | Retinoids (RA) are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. RAs activate the retinoic acid receptor (RAR) and retinoid X receptor (RXR) nuclear transcription factor families and thus modulate the effects of RA on gene expression. Most retinoid forms (including all trans RA, 9-cis RA, 4oxo RA and 3,4 dihydro RA) activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RAR family members, which include RARå, RAR∫ and RAR©, belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. The human RAR∫ gene maps to chromosome 3p25-p21 and encodes two isoforms, RAR∫1 and RAR∫2. The RAR∫2 isoform may act as a tumor suppressor gene by inducing apoptosis. This role for RAR∫2 may explain the chemopreventive and therapeutic effects of retinoids. RAR∫2 expression is diminished or lost completely during breast cancer progression. RAR∫ expression also decreases in over 50 percent of oral and lung premalignant lesions; loss of RAR∫ expression may contribute to carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of RARβ1 and RARβ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of RAR&beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of RARβ of human origin |
| Entrez Gene | 5915 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferus ducts.
Application
Western blot analysis of RARβ expression in U-87 MG whole cell lysate.| Application Notes | recommended for detection of RARβ1 and RARβ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
