Application
Western blot analysis of TCF-4 expression in HeLa nuclear extract.| Product Name | TCF-4 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 486-610 mapping near the C-terminus of TCF-4 of human origin |
| Modification Notes | T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to ∫-catenin in a cooperative manner. This TCF-4/∫-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. c-Jun also interacts with TCF-4 and ∫-catenin, and the phosphorylation-dependent interaction between c-Jun and TCF4 regulates intestinal tumorigenesis by integrating JNK and APC/∫-catenin. TCF-4 is also implicated in bipolar affective disorder. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TCF-4 of human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 486-610 mapping near the C-terminus of TCF-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 486-610 mapping near the C-terminus of TCF-4 of human origin |
| Entrez Gene | 6934 |
|---|
Application
Western blot analysis of TCF-4 expression in HeLa nuclear extract.| Application Notes | recommended for detection of TCF-4 of human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
