Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic staining of pneumocytes and macrophages.Application
Western blot analysis of brachyury expression in A549 whole cell lysate.| Product Name | brachyury Antibody (H-210) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 226-435 mapping at the C-terminus of brachyury of human origin |
| Modification Notes | The T-box gene family consists of members that share a unique DNA binding domain (1). The best characterized T-box (Tbx) gene, Brachyury or T, encodes a transcription factor that plays an important role in early vertebrate development (1). Tbx genes are a family of developmental regulators with more than 20 members recently identified among invertebrates and vertebrates (2). Mutations in Tbx genes have been found to cause several human diseases (2). The understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/Brachyury protein, which is a transcription activator (2). The T-domain is a highly conserved DNA-binding motif originally defined in Brachyury and characteristic of the Tbx family of transcription factors (3). The murine Brachyury (T) gene is required in posterior mesoderm formation and axial development (4,5). Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but develop anterior mesoderm (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of brachyury of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 226-435 mapping at the C-terminus of brachyury of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 226-435 mapping at the C-terminus of brachyury of human origin |
| Entrez Gene | 6862 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic staining of pneumocytes and macrophages.
Application
Western blot analysis of brachyury expression in A549 whole cell lysate.| Application Notes | recommended for detection of brachyury of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
