Application
Western blot analysis of Csk expression in non-transfected 293T: (A), mouse Csk transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Csk expression in non-transfected 293T: (A), human Csk transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Csk expression in AML-193 (A), EOC 20 (B), BC3H1 (C) and CTLL-2 (D) whole cell lysates.Application
Western blot analysis of Csk p50 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human spleen (A) and immunofluorescence staining of methanol-fixed Jurkat cells (B) showing cytoplasmic localization.| Product Name | Csk Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Csk of human origin |
| Modification Notes | All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Csk p50 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Csk of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Csk of human origin |
| Entrez Gene | 1445 |
|---|
Application
Western blot analysis of Csk expression in non-transfected 293T: (A), mouse Csk transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Csk expression in non-transfected 293T: (A), human Csk transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Csk expression in AML-193 (A), EOC 20 (B), BC3H1 (C) and CTLL-2 (D) whole cell lysates.
Application
Western blot analysis of Csk p50 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human spleen (A) and immunofluorescence staining of methanol-fixed Jurkat cells (B) showing cytoplasmic localization.| Application Notes | recommended for detection of Csk p50 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
