Application
Western blot analysis of MEK-4 expression in NIH/3T3 whole cell lysate.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with MEK-4 (C-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Antibodies tested include MEK-4 (K-18): (A,B) and MEK-4 (C-20): (C,D).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and membrane staining.| Product Name | MEK-4 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of MEK-4 of human origin |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of MEK-4 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of MEK-4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of MEK-4 of human origin |
| Entrez Gene | 6416 |
|---|
Application
Western blot analysis of MEK-4 expression in NIH/3T3 whole cell lysate.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with MEK-4 (C-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Antibodies tested include MEK-4 (K-18): (A,B) and MEK-4 (C-20): (C,D).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and membrane staining.| Application Notes | recommended for detection of MEK-4 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
