Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.Application
Western blot analysis of HDAC3 expression in HeLa nuclear extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.Application
Western blot analysis of HDAC3 expression in Jurkat nuclear extract.| Product Name | HDAC3 Antibody (H-99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 330-428 of HDAC3 of human origin |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of HDAC3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 330-428 of HDAC3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 330-428 of HDAC3 of human origin |
| Entrez Gene | 8841 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.
Application
Western blot analysis of HDAC3 expression in HeLa nuclear extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
Application
Western blot analysis of HDAC3 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of HDAC3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
