Application
Cytoplasmic and nuclear proteosomal activator 200 (PA200) in small intestinal adenoma in APC min/+ (20X microscopic magnification). Dilution 1:80 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of PA200 expression in HeLa (A) and A549 (B) whole cell lysates.Application
Western blot analysis of PA200 expression in non-transfected 293T: (A), mouse PA200 transfected 293T: (B), Hep G2 (C), K-562 (D) and HeLa (E) whole cell lysates.| Product Name | PA200 Antibody (A-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of PA200 of human origin |
| Modification Notes | PA200 (proteasome activator 200 kDa), also known as PSME4 (proteasome (prosome, macropain) activator subunit 4), is a 1,843 amino acid nuclear protein that contains six HEAT (Huntington, Elongation Factor 3, PR65/A, TOR) repeats, which are conserved residues that form the hydrophobic domain core and are usually found in proteins that are involved in intracellular transport. Existing as a homodimer, PA200 interacts with the 20S and 26S proteasomes and activates proteasomal cleavage of peptides in an energy-independent manner. PA200 and proteasomes function together within cells and respond to specific radiation-induced damage independent of the stage of cell cycle arrest. Broadly expressed, PA200 may also be involved in spermatogenesis and in DNA repair double-strand breaks (DSBs). Four isoforms of PA200 exists due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PA200 isoforms 1-4 of mouse, rat and human origin by WB, IP, IF , IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of PA200 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of PA200 of human origin |
| Entrez Gene | 23198 |
|---|
Application
Cytoplasmic and nuclear proteosomal activator 200 (PA200) in small intestinal adenoma in APC min/+ (20X microscopic magnification). Dilution 1:80 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of PA200 expression in HeLa (A) and A549 (B) whole cell lysates.
Application
Western blot analysis of PA200 expression in non-transfected 293T: (A), mouse PA200 transfected 293T: (B), Hep G2 (C), K-562 (D) and HeLa (E) whole cell lysates.| Application Notes | recommended for detection of PA200 isoforms 1-4 of mouse, rat and human origin by WB, IP, IF , IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
