Application
Western blot analysis of GPT expression in non-transfected: (A) and mouse GPT transfected: (B) 293T whole cell lysates.| Product Name | GPT Antibody (M-42) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 350-391 mapping near the C-terminus of GPT of mouse origin |
| Modification Notes | The glutamate pyruvate transaminases GPT (or GPT1) and GPT2, also designated alanine aminotransferases (ALT1 and ALT2), respectively, catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT and GPT2 share significant sequence homology, but differ in their expression patterns. GPT exhibits high expression in kidney, liver and heart, whereas GPT2 expression is high in muscle, fat and kidney. GPT is widely used as an index of liver integrity or hepatocellular damage in clinical settings. The genes encoding human GPT maps to chromosome 8q24.3; the gene encoding human GPT2 maps to chromosome 16q12.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GPT of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 350-391 mapping near the C-terminus of GPT of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 350-391 mapping near the C-terminus of GPT of mouse origin |
Application
Western blot analysis of GPT expression in non-transfected: (A) and mouse GPT transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GPT of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
