Application
Western blot analysis of ASK 1 phosphorylation in non-transfected 293T: (A), untreated human ASK 1 transfected 293T: (B) and lambda protein phosphatase treated human ASK 1 transfected: (C) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.| Product Name | p-ASK 1 Antibody (Ser 966) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 966 of ASK 1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| recommended for detection of Ser 966 phosphorylated ASK 1 of human origin and correspondingly phosphorylated Ser 973 of mouse origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 966 of ASK 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 966 of ASK 1 of human origin |
| Entrez Gene | 4217 |
|---|
Application
Western blot analysis of ASK 1 phosphorylation in non-transfected 293T: (A), untreated human ASK 1 transfected 293T: (B) and lambda protein phosphatase treated human ASK 1 transfected: (C) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 966 phosphorylated ASK 1 of human origin and correspondingly phosphorylated Ser 973 of mouse origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
