Application
Western blot analysis of HSF1 expression in non-transfected 293T: (A), human HSF1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HSF1 expression in non-transfected 293T: (A), mouse HSF1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
ChIP analysis of HSF1 binding to promoter regions of HSP 70 (lane 1), HSP 90 (lane 2), Actin (lane 3) and GAPDH (lane 4).Application
Western blot analysis of HSF1 expression in PC-12 (A) and F9 (B) whole cell lysates.| Product Name | HSF1 Antibody (H-311) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 219-529 of heat shock transcription factor 1 (HSF1) of human origin |
| Modification Notes | Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HSF1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 219-529 of heat shock transcription factor 1 (HSF1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 219-529 of heat shock transcription factor 1 (HSF1) of human origin |
| Entrez Gene | 3297 |
|---|
Application
Western blot analysis of HSF1 expression in non-transfected 293T: (A), human HSF1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HSF1 expression in non-transfected 293T: (A), mouse HSF1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
ChIP analysis of HSF1 binding to promoter regions of HSP 70 (lane 1), HSP 90 (lane 2), Actin (lane 3) and GAPDH (lane 4).
Application
Western blot analysis of HSF1 expression in PC-12 (A) and F9 (B) whole cell lysates.| Application Notes | recommended for detection of HSF1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
