Application
Western blot analysis of HURP expression in Ramos whole cell lysate.| Product Name | HURP Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 115-288 mapping near the N-terminus of HURP of human origin |
| Modification Notes | HURP (Hepatoma up-regulated protein), also known as DLGAP5 (Disks large-associated protein 5), DLG7 or DLG1, is an 846 amino acid protein that localizes to both the nucleus and the cytoplasm, specifically localizing to spindle poles in mitotic cells. Expressed in testis, colon, bone marrow, placenta and fetal liver, HURP is thought to function as a cell cycle regulator that interacts with Cdc2 p34 and mediates adherens junction assembly and differentiation in epithelial cells. HURP is upregulated in the G2/M phase of the cell cycle and may play a role in carcinogenesis and tumor transformation via cell cycle control. Upon DNA damage, HURP is phosphorylated by ATM or ATR. Additionally, HURP is subject to ubiquitin-induced proteasomal degradation. Two isoforms of HURP exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HURP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 115-288 mapping near the N-terminus of HURP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 115-288 mapping near the N-terminus of HURP of human origin |
| Entrez Gene | 9787 |
|---|
Application
Western blot analysis of HURP expression in Ramos whole cell lysate.| Application Notes | recommended for detection of HURP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
