Application
Western blot analysis of Rad18 expression in HeLa (A), HEL 92.1.7 (B), K-562 (C), MCF7 (D) and HL-60 (E) nuclear extracts.| Product Name | Rad18 Antibody (H-77) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 8-82 mapping near the N-terminus of Rad18 of human origin |
| Modification Notes | The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rad18 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 8-82 mapping near the N-terminus of Rad18 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 8-82 mapping near the N-terminus of Rad18 of human origin |
| Entrez Gene | 56852 |
|---|
Application
Western blot analysis of Rad18 expression in HeLa (A), HEL 92.1.7 (B), K-562 (C), MCF7 (D) and HL-60 (E) nuclear extracts.| Application Notes | recommended for detection of Rad18 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
