Application
Western blot analysis of Fra-1 expression in non-transfected: (A) and human Fra-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fra-1 expression in 3611-RF (A) and PMA-induced 3611-RF (B) nuclear extracts.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear staining.Application
Western blot analysis of Fra-1 expression in NIH/3T3 nuclear extract.| Product Name | Fra-1 Antibody (N-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Fra-1 of mouse origin |
| Modification Notes | The v-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. Members of the c-Fos gene family, including c-Fos, Fos B, Fra-1 and Fra-2, encode nuclear phosphoproteins that are rapidly and transiently induced by a variety of agents and function as transcriptional regulators for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers that bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Fra-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Fra-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Fra-1 of mouse origin |
| Entrez Gene | 8061 |
|---|
Application
Western blot analysis of Fra-1 expression in non-transfected: (A) and human Fra-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fra-1 expression in 3611-RF (A) and PMA-induced 3611-RF (B) nuclear extracts.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear staining.
Application
Western blot analysis of Fra-1 expression in NIH/3T3 nuclear extract.| Application Notes | recommended for detection of Fra-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
