Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include Fra-2 (L-15): (A) and Fra-2 (Q-20): (B).| Product Name | Fra-2 Antibody (L-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Fra-2 of human origin |
| Modification Notes | The Fos related gene, Fra-2, was initially molecularly cloned from chicken genomic DNA and shown to represent a new member of the immediate early gene family. The human counterpart of the chicken Fra-2 gene has since been described. Sequence alignment shows that the amino acid sequences conserved among Fra-2, c-Fos, Fra-1 and Fos B are contained in five regions. Region 2, the longest and most highly conserved region, contains the leucine zipper structure and the basic region, suggesting that like Fos, Fra-1 and Fos B, Fra-2 also forms heterodimers with c-Jun that recognize a specific DNA sequence such as the binding site for transcription factor AP-1. Such a model is further supported by the finding that the Fra-2 gene product forms a complex with c-Jun in growth-stimulated cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Fra-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Fra-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Fra-2 of human origin |
| Entrez Gene | 2355 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include Fra-2 (L-15): (A) and Fra-2 (Q-20): (B).| Application Notes | recommended for detection of Fra-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
