Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of BAF170 expression in non-transfected: (A) and human BAF170 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of BAF170 expression in non-transfected 293T: (A) and human BAF170 transfected 293T: (B) whole cell lysates and K-562 nuclear extract (C).Application
Western blot analysis of BAF170 expression in A-431 (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of BAF170 expression in A-431 nuclear extract.| Product Name | BAF170 Antibody (H-116) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1093-1208 of BAF170 of human origin |
| Modification Notes | The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila Isw1, a BRM related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of BAF170 of human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1093-1208 of BAF170 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1093-1208 of BAF170 of human origin |
| Entrez Gene | 6601 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of BAF170 expression in non-transfected: (A) and human BAF170 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of BAF170 expression in non-transfected 293T: (A) and human BAF170 transfected 293T: (B) whole cell lysates and K-562 nuclear extract (C).
Application
Western blot analysis of BAF170 expression in A-431 (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of BAF170 expression in A-431 nuclear extract.| Application Notes | recommended for detection of BAF170 of human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
