Application
Western blot analysis of cyclin A expression in HeLa (A), K-562 (B) and HuT 78 (C) whole cell lysates.Application
Western blot analysis of cyclin A expression in untreated (A) and Flurbiprofen treated (B) Caco-2 whole cell lysates. Note down regulation of cyclin A expression in lane B.Application
Blots were probed with cyclin A (H-432): (A), p-cyclin A (Ser 154)-R: preincubated with cognate phosphorylated peptide (B) and p-cyclin A (Ser 154)-R: pre-incubated with cognate unphosphorylated peptide (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.Application
Western blot analysis of cyclin A expression in MCF7 (A), SK-BR-3 (B), A-431 (C), HeLa (D) and U-937 (E) whole cell lysates.| Product Name | cyclin A Antibody (H-432) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-432 representing full length cyclin A of human origin |
| Modification Notes | The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of cyclin A and cyclin A1 of mouse, rat and human origin by WB, IP, KA, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-432 representing full length cyclin A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-432 representing full length cyclin A of human origin |
| Entrez Gene | 890 |
|---|
Application
Western blot analysis of cyclin A expression in HeLa (A), K-562 (B) and HuT 78 (C) whole cell lysates.
Application
Western blot analysis of cyclin A expression in untreated (A) and Flurbiprofen treated (B) Caco-2 whole cell lysates. Note down regulation of cyclin A expression in lane B.
Application
Blots were probed with cyclin A (H-432): (A), p-cyclin A (Ser 154)-R: preincubated with cognate phosphorylated peptide (B) and p-cyclin A (Ser 154)-R: pre-incubated with cognate unphosphorylated peptide (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
Application
Western blot analysis of cyclin A expression in MCF7 (A), SK-BR-3 (B), A-431 (C), HeLa (D) and U-937 (E) whole cell lysates.| Application Notes | recommended for detection of cyclin A and cyclin A1 of mouse, rat and human origin by WB, IP, KA, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
