Application
Western blot analysis of ATF-2 expression in non-transfected 293T: (A), mouse ATF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ATF-2 expression in HeLa whole cell lysate (A) and A-673 (B) nuclear extract.Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.Application
Western blot analysis of ATF-2 expression in non-transfected control (A) and ATF-2 siRNA transfected (B) HeLa cells. Blot probed with ATF-2 (N-96): . Lamin A/C (H-110): used as specificity and loading control.Application
Western blot analysis of ATF-2 expression in K-562 (A) and A-673 (B) nuclear extracts.Application
Antibodies tested include ATF-2 (N-96): (A,B) and p-ATF-2 (Thr 71)-R: (C,D).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing nuclear localization.| Product Name | ATF-2 Antibody (N-96) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-96 mapping at the N-terminus of ATF-2 of human origin |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of ATF-2 of mouse, rat, human and Xenopus origin by WP, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-96 mapping at the N-terminus of ATF-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-96 mapping at the N-terminus of ATF-2 of human origin |
| Entrez Gene | 1386 |
|---|
Application
Western blot analysis of ATF-2 expression in non-transfected 293T: (A), mouse ATF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ATF-2 expression in HeLa whole cell lysate (A) and A-673 (B) nuclear extract.
Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.
Application
Western blot analysis of ATF-2 expression in non-transfected control (A) and ATF-2 siRNA transfected (B) HeLa cells. Blot probed with ATF-2 (N-96): . Lamin A/C (H-110): used as specificity and loading control.
Application
Western blot analysis of ATF-2 expression in K-562 (A) and A-673 (B) nuclear extracts.
Application
Antibodies tested include ATF-2 (N-96): (A,B) and p-ATF-2 (Thr 71)-R: (C,D).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing nuclear localization.| Application Notes | recommended for detection of ATF-2 of mouse, rat, human and Xenopus origin by WP, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
