Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear envelope and cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Lamin A/C expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear envelope staining.Application
Western blot analysis of Lamin A expression in Hs68 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | Lamin A Antibody (H-102) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 563-664 of Lamin A of human origin |
| Modification Notes | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed CED-3/ICE, is comprised of ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3 (ICE-LAP3 or CMH-1), Mch4, and ICE-LAP6. CED-3/ICE family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. Lamin C is a splice variant of Lamin A, differing only at the carboxy terminus. Lamins A and C are identical for the first 566 amino acids, with Lamin C differing only in six unique carboxy-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Lamin A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 563-664 of Lamin A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 563-664 of Lamin A of human origin |
| Entrez Gene | 4000 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear envelope and cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Lamin A/C expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear envelope staining.
Application
Western blot analysis of Lamin A expression in Hs68 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of Lamin A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
