Application
Western blot analysis of USP18 expression in Jurkat whole cell lysate.| Product Name | USP18 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 73-372 mapping at the C-terminus of USP18 of human origin |
| Modification Notes | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. A wide range of enzymes facilitate the proteolytic Ub pathway, including the ubiquitin specific peptidase, USP18 (also designated interferon-stimulated gene 43 (ISG43), ISG15-specific-processing protease, Ubl carboxyl-terminal hydrolase 18 and Ubp43). USP18, a member of the peptidase C19 family, maintains a critical cellular balance of ISG15-conjugated proteins in stressed and healthy organisms. It can efficiently cleave ISG15 fusions including native ISG15 conjugates linked by isopeptide bonds. In mice, deletion of the USP18 gene leads to a large increase of ISG15 conjugates in tissues. USP18 expression is negatively regulated by RNase-L and induced by interferon. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of USP18 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 73-372 mapping at the C-terminus of USP18 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 73-372 mapping at the C-terminus of USP18 of human origin |
| Entrez Gene | 11274 |
|---|
Application
Western blot analysis of USP18 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of USP18 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
