Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and human Cdk7 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Cdk7 expression in K-562 (A), Jurkat (B), HeLa (C) and NIH/3T3 (D) nuclear extracts.Application
Western blot analysis of Cdk7 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and mouse Cdk7 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and human Cdk7 transfected: (B) 293 whole cell lysates.Application
Antibodies tested include Cdk7 (N-19): (A,B) and Cdk7 (FL-346): (C,D).| Product Name | Cdk7 Antibody (FL-346) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-346 representing full length Cdk7 of human origin |
| Modification Notes | Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of Cdk7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-346 representing full length Cdk7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-346 representing full length Cdk7 of human origin |
| Entrez Gene | 1022 |
|---|
Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and human Cdk7 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Cdk7 expression in K-562 (A), Jurkat (B), HeLa (C) and NIH/3T3 (D) nuclear extracts.
Application
Western blot analysis of Cdk7 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and mouse Cdk7 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdk7 expression in non-transfected: (A) and human Cdk7 transfected: (B) 293 whole cell lysates.
Application
Antibodies tested include Cdk7 (N-19): (A,B) and Cdk7 (FL-346): (C,D).| Application Notes | recommended for detection of Cdk7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
