Application
Western blot analysis of SPRED1 expression in non-transfected (A) and human SPRED1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Product Name | SPRED1 Antibody (Y-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SPRED1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | SPRED1 (sprouty-related, EVH1 domain containing 1), also known as NFLS, is a 444 amino acid protein that contains one KBD domain, one sprouty domain and one WH1 domain. Localized to the cell membrane and to cholesterol-rich membrane raft fractions, SPRED1 functions as a tyrosine kinase that regulates the activity of the ERK (also known as MAP kinase) cascade by inhibiting the growth-factor-mediated activation of ERK. SPRED1 can act independently as a homodimer or can function as a heterodimer with SPRED2 and, in addition to its ability to regulate ERK, is thought to negatively regulate the development of blood cells in bone marrow. Defects in the gene encoding SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS), an autosomal dominant disease that is characterized by multiple cafe-au-lait spots, axillary freckling and macrocephaly. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SPRED1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SPRED1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 161742 |
|---|
Application
Western blot analysis of SPRED1 expression in non-transfected (A) and human SPRED1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
