| Product Name | Siglec-12 Antibody (X-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Siglec-12 of human origin by IF, IHC(P) and ELISA |
| Modification Notes | Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated Siglecs, are immunoglobulin superfamily members that recognize sialylated ligands. Siglecs are important in the functions of the haemopoietic, immune and nervous systems. Siglec-12, also designated Siglec-L1, is expressed on the lumenal edge of epithelial cell surfaces. The Siglec-12 transcript is alternatively spliced, resulting in a short and long isoform. Siglec-12 lacks a conserved arginine residue known to be necessary for optimal sialic acid recognition by other previously known Siglecs. This arginine loss from an ancestral molecule was caused by a single nucleotide substitution that occurred between the common ancestor of humans with the great apes, and the origin of modern humans. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Siglec-12 of human origin by IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Siglec-12 of human origin by IF, IHC(P) and ELISA |
| Entrez Gene | 89858 |
|---|
| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
