Application
Western blot analysis of TAF II p28 expression in SK-N-MC nuclear extract.Application
Western blot analysis of human TAF II p28 transfected 293T whole cell lysate.| Product Name | TAF II p28 Antibody (S-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TAF II p28 of human origin by WB, IP and ELISA |
| Modification Notes | TFIID is a general transcription factor that facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. TFIID is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). The TAF II family members include p18, p28, p32, p100, p130, p170 and p250, which is the largest subunit of TFIID. TAF II p32 is the human homologue of the Drosophila TAFII40 and is upregulated during apoptosis. TAFII p32 interacts with the activation domain of the viral protein 16, TFIIB and the class II transactivator (CIITA) to modulate transcription. The human and murine TAFII p32 proteins are distinct isoforms, designated TAF II p32 a and b, respectively, and they are thought to have individual roles in regulation. TAF II p28 and TAF II p18 interact with one another in vitro and intracellularly, and both interact with TBP through distinct domains. TAF II p28 potentiates transactivation of the estrogen and vitamin D3 receptors (ER and VDR), and is the limiting factor in the RXRa activation pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TAF II p28 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TAF II p28 of human origin by WB, IP and ELISA |
| Entrez Gene | 6882 |
|---|
Application
Western blot analysis of TAF II p28 expression in SK-N-MC nuclear extract.
Application
Western blot analysis of human TAF II p28 transfected 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
