Application
Western blot analysis of NRIP expression in human muscle tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse muscle (C) tissue extract and rat muscle (D) tissue extract.| Product Name | NRIP Antibody (F-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NRIP of mouse, rat and human origin by WB and IP; also reactive with additional species, including canine |
| Modification Notes | WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. NRIP (Nuclear receptor interaction protein), also known as IQWD1 ( IQ motif and WD repeat-containing protein 1), MSTP055, ARCAP or PC326, is an 860 amino acid protein that localizes to the nucleus and contains one IQ domain and seven WD-repeats. Expressed in testis, skeletal muscle, prostate and heart, NRIP functions as a ligand-dependent coactivator of nuclear receptors and specifically enhances the transcriptional activity of AR (androgen receptor) and GR (glucocorticoid receptor). NRIP exists as three isoforms that are produced by alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NRIP of mouse rat and human origin by WB and IP also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of NRIP of mouse, rat and human origin by WB and IP; also reactive with additional species, including canine |
| Entrez Gene | 55827 |
|---|
Application
Western blot analysis of NRIP expression in human muscle tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse muscle (C) tissue extract and rat muscle (D) tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
