Application
Western blot analysis of VR1 expression in non-transfected: (A) and mouse VR1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of VR1 expression in non-transfected: (A) and human VR1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of VR1 expression in HeLa (A), heat shock induced HeLa (B) and THP-1 (C) whole cell lysates.| Product Name | VR1 Antibody (R-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 mapping at the N-terminus of VR1 of rat origin |
| Modification Notes | Vanilloid receptor 1 (VR1), also designated capsaicin receptor, is a nonselective cation channel, structurally related to members of the TRP family of ion channels. VR1 is activated by capsaicin, the active ingredient in chili peppers, by heat and by an increase in protons at sites of infection, inflammation and ischemia. By creating moderately acidic conditions, protons are able to lower the temperature threshold for VR1 activation, thus identifying VR1 as a molecular integrator of chemical and physical stimuli that elicit pain. VR1 is expressed in primary sensory neurons and vagal nerves and activated VR1 induces the influx of cations, particularly Ca2+ and Na+ ions. The vanilloid receptor may also be a molecular target for endogenous anandamide, in addition to the cannabinoid receptors, in the nervous and cardiovascular systems. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VR1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 mapping at the N-terminus of VR1 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 mapping at the N-terminus of VR1 of rat origin |
| Entrez Gene | 7442 |
|---|
Application
Western blot analysis of VR1 expression in non-transfected: (A) and mouse VR1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of VR1 expression in non-transfected: (A) and human VR1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of VR1 expression in HeLa (A), heat shock induced HeLa (B) and THP-1 (C) whole cell lysates.| Application Notes | recommended for detection of VR1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
