Application
Western blot analysis of JMJD2D expression in human fetal brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and membrane localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.| Product Name | JMJD2D Antibody (G-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of JMJD2D of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | JMJD2D (Jumonji domain-containing protein 2D), also known as JHDM3D or KDM4D, is a 520 amino acid protein that belongs to the JHDM3 histone demethylase family. Localized to the nucleus, JMJD2D functions as a histone demethylase that removes specific methyl residues from Histone H3, thereby playing a crucial role in the histone code. JMJD2D binds iron as a cofactor and contains one JMJC domain and one JMJN domain, both of which are thought to exhibit enzymatic activity during chromatin remodeling events. In addition, JMJD2D forms a complex with the ligand-bound form of the androgen receptor (AR) and, through this interaction, activates AR expression. Overexpression of AR is associated with prostate cancer, suggesting that, via its ability to upregulate AR, JMJD2D may be involved in carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of JMJD2D of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of JMJD2D of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 55693 |
|---|
Application
Western blot analysis of JMJD2D expression in human fetal brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and membrane localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
