Application
Western blot analysis of TCR α expression in non-transfected: (A) and human TCR α transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TCR α expression in K-562 (A) and HuT-78 (B) whole cell lysates.| Product Name | TCR α Antibody (H-142) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to the constant region of TCR α of human origin |
| Modification Notes | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either å and ∫ or © and ∂ chains. The vast majority of circulating T cells (95%) express the å/∫ heterodimer while roughly 2-5% express the ©/∂ heterodimer. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8. T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4, also designated T4 and Leu 3, is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR, in association with CD4, can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TCR α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to the constant region of TCR &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to the constant region of TCR α of human origin |
| Entrez Gene | 6955 |
|---|
Application
Western blot analysis of TCR α expression in non-transfected: (A) and human TCR α transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TCR α expression in K-562 (A) and HuT-78 (B) whole cell lysates.| Application Notes | recommended for detection of TCR α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
