Application
Western blot analysis of GIOT-3 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.| Product Name | GIOT-3 Antibody (P-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GIOT-3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GIOT-3 (gonadotropin-inducible ovary transcription repressor 3), also known as ZNF12 (zinc finger protein 12), ZNF325 (zinc finger protein 325), KOX3 or HZF11, is a 501 amino acid nuclear protein belonging to the Krüeppel C2H2-type zinc-finger protein family. GIOT-3 is suggested to play a role in transcriptional regulation of MAPK signaling pathways, thereby mediating cellular functions. Containing eight C2H2-type zinc fingers and a KRAB domain, GIOT-3 is encoded by a gene that maps to human chromosome 7p22.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GIOT-3 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GIOT-3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 7559 |
|---|
Application
Western blot analysis of GIOT-3 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
