Application
Western blot analysis of TRIM10 expression in Hep G2 whole cell lysate.Application
Western blot analysis of TRIM10 expression in 293T whole cell lysate.| Product Name | TRIM10 Antibody (F-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRIM10 of human origin by WB, IP and ELISA |
| Modification Notes | The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM10, also known as RING finger protein 9, RFB30 or HERF1, is a 481 amino acid protein that localizes to the cytoplasm. Expressed exclusively in hematopoetic tissues that contain developing myeloid, erythroid or megakaryocytic progenitors, TRIM10 has been shown to play a critical role in the terminal differentiation of erythroid cells. The functions of the various domains in TRIM10 suggest a role in the regulation of transcriptional signaling as well a mechanistic role in the morphological changes that occur during erythroid development. The expression of TRIM10 is dependent on upstream effectors such as PEBP2∫ and PU.1. Two named isoforms of TRIM10 exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRIM10 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRIM10 of human origin by WB, IP and ELISA |
| Entrez Gene | 10107 |
|---|
Application
Western blot analysis of TRIM10 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of TRIM10 expression in 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
