Application
Western blot analysis of Msx-1 expression in SP2/0 whole cell lysate.| Product Name | Msx-1 Antibody (F-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Msx-1 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Msx homeobox genes encode for transcription factors that control morphogenesis and are expressed at sites of epithelial-mesenchymal interaction during embryogenesis, such as the tooth (1,2). Two of these genes, Msx-1 and Msx-2, are key factors for the development of tooth and craniofacial skeleton (2,3). Msx-1 also down-regulates a master gene of skeletal cells differentiation (3). Msx-1 and Msx-2 contribute to the initial patterning of dentition as well as playing a pivotal role in terminal cell differentiation (1). In addition, Msx-1 and Msx-2 are expressed in the epidermis, hair follicles and fibroblasts of the developing fetal skin (4). In adult skin, Msx-1 and Msx-2 expression is confined to epithelially derived structures (4). Msx-2 is detected as a diffuse cytoplasmic signal in fetal epidermis and portions of the hair follicle and dermis, but is localized to the nucleus in the adult epidermis (4). Msx-1 and Msx-2 are also expressed during critical developmental stages of neural tube and neural crest, suggesting that these genes play an important role in organogenesis (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Msx-1 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Msx-1 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 4487 |
|---|
Application
Western blot analysis of Msx-1 expression in SP2/0 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
