Application
Western blot analysis of RNF8 expression in non-transfected: (A) and human RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.Application
Western blot analysis of RNF8 expression in non-transfected: (A) and human RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.Application
Western blot analysis of RNF8 expression in non-transfected: (A) and mouse RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.Application
Western blot analysis of RNF8 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Product Name | RNF8 Antibody (X-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RNF8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The RING finger motif is a specialized DNA-binding zinc finger domain found in many transcriptional regulatory proteins. The ring finger protein (RNF) family includes any protein containing the signature RING finger motif. RNF8 is a ubiquitously expressed nuclear RING finger protein that acts as an E3 ubiquitin-protein ligase. It is required for the ubiquitination of some nuclear proteins and promotes their subsequent degradation. The heterodimeric ubiquitin-conjugating enzyme UBC13 interacts with RNF8, and they co-localize in the nucleus. RNF8 may regulate mediation of UBC13 polyubiquitylation by elongating the ubiquitin chains. RNF8 also binds to Retinoid X receptor alpha (RXRå), a member of the steroid hormone receptor superfamily. It increases RXRå-mediated transactivation of the RXRå-responsive element (RXRE) promoter in a dose-dependent manner, suggesting that RNF8 is a regulator of RXRå-mediated transcriptional activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RNF8 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RNF8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9025 |
|---|
Application
Western blot analysis of RNF8 expression in non-transfected: (A) and human RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.
Application
Western blot analysis of RNF8 expression in non-transfected: (A) and human RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.
Application
Western blot analysis of RNF8 expression in non-transfected: (A) and mouse RNF8 transfected: (B) 293T whole cell lysates and Jurkat (C) and THP-1 (D) nuclear extracts.
Application
Western blot analysis of RNF8 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
