Application
Western blot analysis of H+/K+ ATPase β expression in non-transfected: (A) and human H+/K+ ATPase β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of H+/K+ ATPase β expression in non-transfected: (A) and human H+/K+ ATPase β transfected: (B) 293 whole cell lysates.| Product Name | H+/K+ ATPase β Antibody (D-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an extracellular domain of H+/K+ ATPase β of human origin |
| Modification Notes | The gastric H+/K+ ATPase exists as a heterodimer consisting of an å and a ∫ subunit that work in tandem to transport protons across plasma membranes. H+/K+ ATPase ∫, also known as ATP4B or ATP6B, is a 291 amino acid single-pass type II membrane protein that functions as the ∫ subunit of the H+/K+ ATPase heterodimer. Working with the å subunit, H+/K+ ATPase ∫ effectively catalyzes the the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane and plays an essential role in gastric acid secretion. The gene encoding H+/K+ ATPase ∫ maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of H+/K+ ATPase β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an extracellular domain of H+/K+ ATPase &beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an extracellular domain of H+/K+ ATPase β of human origin |
| Entrez Gene | 496 |
|---|
Application
Western blot analysis of H+/K+ ATPase β expression in non-transfected: (A) and human H+/K+ ATPase β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of H+/K+ ATPase β expression in non-transfected: (A) and human H+/K+ ATPase β transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of H+/K+ ATPase β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
