Application
Western blot analysis of eIF2Bα expression in non-transfected: (A) and mouse eIF2Bα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of eIF2Bα expression in non-transfected: (A) and human eIF2Bα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of eIF2Bα expression in non-transfected 293T: (A), mouse eIF2Bα transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | eIF2Bα Antibody (FL-305) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-305 representing full length eIF2Bα of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bå, eIF2B∫, eIF2B©, eIF2B∂ and eIF2Bε. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity is exhibited by the eIF2Bε subunit alone, but is greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bå subunit. Defects in the gene encoding eIF2Bå are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease that is characterized by head trauma and motor deterioration. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF2Bα of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-305 representing full length eIF2B&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-305 representing full length eIF2Bα of human origin |
| Entrez Gene | 1967 |
|---|
Application
Western blot analysis of eIF2Bα expression in non-transfected: (A) and mouse eIF2Bα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of eIF2Bα expression in non-transfected: (A) and human eIF2Bα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of eIF2Bα expression in non-transfected 293T: (A), mouse eIF2Bα transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of eIF2Bα of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
