Application
Western blot analysis of Dim1 expression in non-transfected: (A) and mouse Dim1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Dim1 expression in rat brain tissue extract.| Product Name | Dim1 Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Dim1 of human origin |
| Modification Notes | The Dim protein family consists of two classes, Dim1 and Dim2, which share a common thioredoxin-like fold, but most likely function in different biological pathways. Dim1, also known as TXNL4A (Thioredoxin-like protein 4A) or Spliceosomal U5 snRNP-specific 15 kDa protein, is a 142 amino acid protein that plays an essential role in pre-mRNA splicing. Due to a failure to express early zygotic transcripts, deletion of the gene encoding Dim1 in Schizosaccharomyces pombe leads to embryonal lethality during gastrulation. Since Dim1 shows sensitivity to a microtubule destabilizing drug, thiabendazole, it also may play a role in the formation of the mitotic spindle. This evidence demonstrates that Dim1 is essential for G2/M progression of the cell cycle and chromosomal segregation during mitosis. Localized to the nucleus, Dim1 interacts with hnRNP F, hnRNP H2, Cas-L and PQBP-1 to effect gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dim1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Dim1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Dim1 of human origin |
| Entrez Gene | 10907 |
|---|
Application
Western blot analysis of Dim1 expression in non-transfected: (A) and mouse Dim1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Dim1 expression in rat brain tissue extract.| Application Notes | recommended for detection of Dim1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
