Application
Western blot analysis of SCYL2 expression in non-transfected: (A) and mouse SCYL2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SCYL2 expression in T47D (A) and mouse brain (B) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining.| Product Name | SCYL2 Antibody (C-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SCYL2 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | SCYL2 (SCY1-like 2), also known as CVAK104 (Coated vesicle-associated kinase of 104 kDa), is a 929 amino acid protein that contains one HEAT repeat and one protein kinase domain. Localized to a variety of locations within the cell, including the perinuclear region of the cytoplasm, the trans-golgi network membrane and calthrin-coated vesicles, SCYL2 exists as a component of AP2-containing Clathrin coated structures. SCYL2 is thought to function as a Ser/Thr protein kinase, exhibiting kinase activity towards adaptor complex proteins, possibly regulating Clathrin-dependent trafficking events between the golgi network and endosomes. Additionally, SCYL2 may play a role in the sorting of SNARE proteins and, ultimately, in embryonic development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SCYL2 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SCYL2 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 55681 |
|---|
Application
Western blot analysis of SCYL2 expression in non-transfected: (A) and mouse SCYL2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SCYL2 expression in T47D (A) and mouse brain (B) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
