Application
Western blot analysis of p-IRAK-1 expression in Ramos tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-IRAK-1 Antibody (Ser 376) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 376 phosphorylated IRAK-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification | Phosphoralated |
| Modification Notes | Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1a and IL-1b, and a specific receptor antagonist, IL-1Ra. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. The IL-1-dependent kinase termed IRAK-1 (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFkB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkB. Human IRAK-1 is subject to autophosphorylation on specific amino acid residues, such as Ser 376. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| See 1 citations for p-IRAK-1Antibody (Ser 376): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 376 phosphorylated IRAK-1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 376 phosphorylated IRAK-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 3654 |
|---|
Application
Western blot analysis of p-IRAK-1 expression in Ramos tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | See 1 citations for p-IRAK-1Antibody (Ser 376): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
