Application
Western blot analysis of ZNF265 expression in non-transfected: (A) and human ZNF265 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ZNF265 expression in HeLa whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining.| Product Name | ZNF265 Antibody (E-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against ZNF265 of human origin |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF265 (Zinc finger protein 265), also known as ZRANB2 (Zinc finger Ran-binding domain-containing protein 2), ZIS, ZIS1 or ZIS2, is a 330 amino acid protein that belongs to the ZRANB2 family. Localized to the nucleus, ZNF265 functions as a splicing factor that is responsible for alternatively splicing Tra-2∫ (transformer-2 beta) transcripts and is thought to interfere with constitutive 5’-splice selection. ZNF265 contains two RanBP2-type zinc fingers through which it conveys its RNA-binding activity. Two isoforms, designated ZIS-1 and ZIS-2, are expressed due to alternative splicing events. Upon DNA damage, ZIS-2 may be phosphorylated by ATM or ATR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ZNF265 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ZNF265 of human origin |
|---|---|
| Tissue Specificity | raised against ZNF265 of human origin |
| Entrez Gene | 9406 |
|---|
Application
Western blot analysis of ZNF265 expression in non-transfected: (A) and human ZNF265 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ZNF265 expression in HeLa whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining.| Application Notes | recommended for detection of ZNF265 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
