Application
Western blot analysis of ICMT expression in T4D7D (A) and SK-BR-3 whole cell lysate (B).Application
Western blot analysis of ICMT expression in 293T (A) and HeLa (B) whole cell lysates.| Product Name | ICMT Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ICMT of human origin by WB, IP and ELISA |
| Modification Notes | ICMT (isoprenylcysteine carboxylmethyltransferase), also known as PCCMT, HSTE14 or PPMT, is a 284 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and belongs to the isoprenylcysteine O-methyltransferase family. Expressed ubiquitously with highest expression in brain tissue, ICMT uses divalent cations (such as zinc) to catalyze the post-translational methylation of isoprenylated C-terminal cysteine residues. Specifically, ICMT functions as the third of three enzymes that modify isoprenylated C-terminal cysteine residues on target proteins, thereby targeting those proteins to the cell membrane. Inhibition of ICMT effects Ras function and causes endothelial apoptosis, an event that may lead to a decrease in oncogenic transformation. Multiple isoforms of ICMT exist due to alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ICMT of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ICMT of human origin by WB, IP and ELISA |
| Entrez Gene | 23463 |
|---|
Application
Western blot analysis of ICMT expression in T4D7D (A) and SK-BR-3 whole cell lysate (B).
Application
Western blot analysis of ICMT expression in 293T (A) and HeLa (B) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
